This study evaluates targeted AI tools in BIOL 3451 (Genetics) at UNT Dallas using identical content across semesters, including lectures, textbooks, and assignments. Experimental sections used two AI interventions: chapter-specific custom chatbots to answer any questions and Character AI personas to role-play topics such as DNA replication, Mendelian inheritance, and population genetics. Control sections had no AI access. Data from over 100 students shows an average 30% increase in assignment scores among AI users. Student feedback reports improved confidence, retention, and engagement, with ethical guardrails requiring disclosure and prohibiting AI-generated submissions, demonstrating scalable, low-cost personalized tutoring.#Chatbot #AI Charchter # Teaching